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KMID : 0613820020120020219
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Journal of Life Science
2002 Volume.12 No. 2 p.219 ~ p.225
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Characterization of a New Gene Resistant to Alkylating Agents and 3-Aminobenzamide When Knocked Out in Fission Yeast
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Cha Jae-young
Hwang Sung-Jin
Park Se-Guen
Kim Mi-Young
Baek Sung-Min
Choi In-Soon
Lee Jung-Sup
Park Jong-Keun
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Abstract
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The organization of eukayotic chromatin into specific conformation that are associated with transcription, replication, reapir and other nuclear processes are achieved via a series of DNA-protein interaction. These interactions are mediated by a range of DNA-binding domains such as SAP domain et al. By searching S. pombe genomic DNA database, we have found a gene named SAPuvs (SAP UV Sensitive) whose amino acid sequence is in part similar to SAP domain of Arabidopsis poly (ADP-ribose) polymerase and Ku70. Knock-out cell of S. pombe SAPuvs gene was constructed using Ura4 as a selection marker. Survival analysis of knock-out cell indicated that treatment with UV significantly reduces the survival compared to wild type cell. Potentiation of MMS-induced cytotoxicity by 3AB post-treatment was observed in wild type cells, but not in knock-out cells. These data suggested that the protein encoded by SAPuvs gene is associated with chromatin reorganization during DNA repair.
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KEYWORD
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S. pombe, MMS (methylmethane sulfonatde), UV, 3AB (3-Aminobenzamide), PARP (poly (ADP-ribose)polymerase), SAP (SAF-A/B, Acinus and P-IAS) domain
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